Where oh where has my mg gone?

Yeah I considered it myself yet the volume feels surprisingly high.

I remember someone mentioning that filters always keep back a little liquid. The smaller size holds roughly five portions and the bigger one holds fifteen. On top of that the holders still contain some at the finish because they narrow toward the bottom and the rod stops short of reaching it. Maybe that explains why the figures come out uneven.

I confirmed using the 13mm .22um filter. Hadn't noticed fifteen units still inside. The ten milligram portion measured out to forty five units while the thirty four milligram came to fifty three. It surprised me when the vial emptied after just three uses. Probably better to pick the higher concentration ones going forward, heh.

A comparable issue occurred for me while attempting to prepare the mixture. Fluid escaped past the filter, and because I'm so impatient, I sampled it with my tongue. Were you drawing the syringe back to send air across the filter and return the fluid to the bottle? These figures just don't add up for me. Apologies.

That shortfall still seems odd and I feel the same way about it. I figure the other pieces probably stayed behind in the basket as well, funny how things go.

I remain using that identical package of those small needles. I looked into the details myself. The amount that stays behind reaches as high as twenty nine units, listed between ten and twenty nine. This accounts for much of it. The other portion likely came from the initial draw plus whatever remained inside the container.

My lecture just ended so I can focus properly now. Please lay out the details clearly. A ten milligram container of Reta got mixed together using one milliliter of BAC water leaving a single milliliter solution holding ten milligrams per milliliter. What came next after that?

10 mg vial, added 1 ml H-bac so 1 mg per 10 units

I perform that priming step solely at the start so trapped air and bubbles can escape.

When you filter again the second time, does that initial amount get released then? So isn't the only loss those maximum 28 units just once from the final push?

yes thats right. Now what happened after this reconstitution?

No clue here either, chuckles. Likely the filter snatched it up somehow. Still, that extra charge from the filter works out fine for me.

Filtering fails to get rid of endotoxins at all. Doing it a second time just uses up materials for nothing and might introduce more impurities instead. Those toxins are way too tiny for any standard filter to catch. I've heard about special charged filters that could possibly handle them though. Are you relying on one of those? Without that, repeating the filtration step raises the chance of problems rather than lowering it.

The amount lost, 45 units from those vials, exceeds what normally gets left in a filter. There could be other reasons for this. Maybe the device delivers an incorrect amount each time. Perhaps mistakes in counting the doses given out, though that seems improbable here given how straightforward the calculation is. Or possibly the tool used to measure the liquid isn't accurate, but I doubt that's it. Between those options, the initial one looks most believable to me. Which device are you employing, and did you check its accuracy?

That take really clicks for me. We'd probably vibe nicely together. I nibble the stalks as I load the pipe.

capitalism FTW...we OWN the vial

That cracked me up. The poster ought to look into ways of cutting down lost solution from the filter. Pushing air through reclaims a portion, rinsing pulls back even more, and soaking it first keeps the biggest share. The problem is most of those steps call for an added needle, though the air step skips that. Options still exist to limit extra needles, such as using a low-cost insulin syringe for the initial soak. Ordering needles by the larger lot brings the per-unit cost way down. Separately, testing the dosing device itself for consistency might be worthwhile. I once owned a unit bought online that gave erratic amounts, mostly short but now and then over. A helpful clip explains how to check the device.

I wonder whether these items really do anything useful at all, above all with a budget version, yet keeping one on hand makes me feel better since there is a chance it could make some difference.

Perhaps the filter played a role, or an inaccurate measurement led to some loss on my part. Three milligrams seems like a significant dose.

Grateful for the tip, learned about clearing extras only after counting my existing items recently. Next round I'll handle that for sure. Normally process two or three units together, though previously did extra because freezing works for this compound. Purchased a large amount so now hold more than a hundred with plenty left over. Verified multiple occasions using various amounts. Getting larger quantities sooner would likely mean greater reduction, funny enough. Thanks again!

It's wild this still goes down even using bottles plus needles 🤷‍♀️

Initially suspected the filter too, yet now suspect the loss might tie to those insulin syringes or the sterile vials in some manner. We split one kit between my best friend, my husband, and myself, so the three of us handled reconstitution as a group. Asked her to double-check the amounts. Because our area is fairly rural and she preferred not to carry pre-filled syringes on the drive, I measured her share for the full month, she verified it again, then I transferred everything into a fresh sterile vial. She came up ten units short and I came up twenty short. Will switch to different syringes on the next round to check whether that changes anything.

Yeah that matched my own thinking since my figures checked out properly though the whole thing left me puzzled.

This seems wild. Vapor loss might explain it? Nothing else occurs to me as another option.

My guess is your eyes stayed on the handle section instead of the front end while mixing the solution.

Does that mean running bac water across the filter first prior to putting the mixed solution through it?

True, that makes sense. A filter that's not moistened first would soak up the mixed peptide solution, whereas wetting it beforehand lets the solution flow through more freely since the filter takes in the liquid instead. These days I skip that step entirely along with rinsing the filter afterward. All I do now is blow air through it once finished. It's just not worth the extra effort for the small gains. No one's ever posted a comparison showing recovery rates from air blowing compared to rinsing or soaking ahead of time, though I wonder what the numbers would show.

I'm unsure as well. That doesn't seem right to me, given my two decades as a nurse means I'm familiar with handling these items. The filters stood out as the unfamiliar part. My close friend verified the amounts for me. The mixing day arrives soon, so I'll tweak a few aspects and check the outcome then.