Supply list and vent needle question

I use 5/8 inch luer lock needles for drawing bacteriostatic water, reconstitution, and venting. I follow the steps shown in this video.

I picked up some half inch lockable needles without any syringe and used them to release air from the fresh sterile container during filtering.

It is really whatever you have access to. If you use pens then a pen needle works fine to vent. Otherwise just use a fresh reconstitution needle.

I spotted what looks like trouble around the expression coring so figured I would share this reading on how vials can shatter into fragments along with that process being preventable from a research archive and a video clip covering it.

I have been thinking about this too. There is no such thing as a special vent needle, correct? It is just a regular needle that is used for venting. I believe on that same video he says he uses 27 gauge. Does that sound right?

I learned that certain needles get made just for releasing pressure in transfers like this. Choosing one that's oversized can punch out pieces of the stopper while an undersized version tends to clog up fast. Online lookups suggested gauges from 21 through 25 handle the job best.

Rather than relying on a dedicated vent needle I first draw back the plunger to match the fluid amount with air then push that into the water vial before pulling out the liquid to keep pressure even. After that I put the needle into the peptide container and suck out half a milliliter of air then switch between adding liquid and removing air in small equal steps until the move is done. Before pulling the needle I let the plunger rest on its own and if any empty space shows up I fill it first so the container stays balanced.

Grateful for the info! As far as the needles go the maker isn't a huge concern but a trusted filter together with the bottles seems preferred am I getting that right?

For venting I just grab inexpensive ones. Someone gave me a huge stack of insulin syringes so I pop the plunger and repurpose those. No point adding a filter to the vent. On mixing I agree that longer needles work better since they reach the bottom and let you recover everything. For clearing the filter there are a few ways to handle it. Skip the step and accept losing some liquid depending on filter size, push air through to recover part of it, or sacrifice another syringe to run extra water and flush most of the remaining material. I have never tried the smallest filters myself but opinions vary on whether they handle a full three-milliliter mix without issues, though many say they manage fine. One extra step that might cut down on waste is soaking the filter first with some liquid before the main pass. I do that using one of the free syringes I already mentioned.

Something to watch.

I enjoy putting a filter on the air vent needle too. It seems logical since I'm already cleaning out all the other materials entering my vial, which happens to be a cartridge in my case. Why not reduce the tiny particles like bacteria or spores that come from ordinary household air as much as possible. Doing this in a sterile environment would be different, yet here I am working from the kitchen instead.

A face covering cuts down on particles getting into the mix while I'm putting the solution together. It's inexpensive and straightforward, and by now the habit feels normal. I skip it when actually injecting unless the alcohol wipes trigger a sneeze, which happens to me. Gloves aren't mandatory, yet if I put them on I wipe them down with sanitizer first. Some routines stopped recommending them a couple of years back because people lose track of every surface their hands brush against. Little motions like rubbing an itch, shifting a seat, or grabbing something that never got wiped can slip by unnoticed when you're on your own. Bare hands make those slips more obvious, and washing up again only takes half a minute once you know how the gel with aloe feels. Drafts from open windows, moving air, or the AC unit add another risk I try to limit. Since I shed arm and scalp hair easily I pull on long sleeves and something over my head. The kitchen scrap bucket with used coffee also brings in mold spores that grow fast in damp grounds. After watching two friends accidentally swap bottles and splash acid during a mix-up I started wearing eye protection every time. Getting even a little of the preservative liquid in your eye is something I'd rather avoid.

A well known brand has 13 mm PES 0.22 um filters in larger packs. Solid brand that keeps me filtered for almost a year since I am the reconstitutor for a handful of people.

Following up on what I said before, those 4mm ones weren't available then. The bigger 13mm versions show up more often and look simpler to locate. Having extra choices plus packs with fewer units works well for me. I go through roughly a hundred each year.

Very interesting. I am prone to use 4 mm after this video because I will likely use 2 ml.

I'm unsure if this method is correct, yet I place a small syringe needle into the container while the mixed liquid and filtering device are already there. Pushing the solution slowly via the filter into the bottle causes the syringe's plunger to move upward on its own. Now and then I give it a light assist to balance the internal air pressure. Once fully extended I remove the needle, push the plunger back down to reset it, and insert again.