Users report noticeable volume reduction after pushing reconstituted peptide through a 4 mm filter into a new vial. One participant attributes the discrepancy to unit versus microliter measurements and cites a 1 µL hold-up spec, while another references a video claiming 7 units. Several people describe practical steps such as drawing air before filtering or simply pushing through without extra measures, with mixed results on minimizing loss.
What this discussion establishesWhether the hold-up volume of a 4 mm filter is closer to 7 units or only 1 µL.
Still openExact volume retained by the specific filter in real-world use and the most reliable way to recover it.
Nothing here is advice.
When I add 3 ml of bac water to a 3 ml peptide it looks full, but after drawing it out, attaching a filter, and moving it to a new vial I end up about a quarter short. Some liquid is obviously staying in the filter. Am I doing this right? What does everyone else see?
How big is your filter? A video says the 4 mm filter holds about 7 units.
The hold-up is likely µL versus units; 7 units would be a huge amount. The site lists a hold-up volume of 1 µL for that filter.
I dunno, I went by what the filtering video said around the five-minute mark. I'm no expert though.
I grab it quick and take it in. My system sorts everything out on its own haha
I take in a small breath after removing the pep but ahead of securing the filter. That step ensures it ends up fully cleared. It succeeds on about three fifths of the tries, without fail each time.
Blowing air through changes how much liquid stays trapped behind. I follow that method and end up with almost no waste.
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