Do you wet your filter?

Can't you just wet it using bac water? Draw 3 ml bac into a syringe, put 1 ml into the peptide vial, send 1 ml through the filter into a donor vial, pull the reconstituted peptide into another syringe and push it through the filter, then use the last ml of bac to rinse the filter.

Good question, I just got some filters myself. Pushing the liquid through was tougher than I expected.

Yeah, my venting filter needle stopped working on one vial and I lost 70 mg of glow.

I tried the shorter needles for venting several vials and they must have dulled by the time I reached the pen cartridge. Now I just filter straight into the final cartridge the way the videos show, instead of filtering the bac and the source vial.

Yep, saved a dollar on two filters and lost a nineteen-dollar vial.

Air couldn't escape through the vent needle. When I pushed the glow in through the other filter needle the rubber plunger popped out and everything leaked.

The only reason I can see for wetting a filter is to fill the empty space inside it with liquid. When dry it can hold onto 5-15 units like a sponge and throw off your dosing. Try practicing with bac water on an old vial first. I have never had a filter break or clog in a year of use. I stick with PES 0.22 µm 13 mm.

Yes I do.

I wet coffee filters for pour-over, backwash the pool sand filter when changing media, and flush the sediment and charcoal filters on my water system. So I plan to wet the peptide filter too. I want it to act as a one-way filter, so my idea is to draw bac water into the syringe, attach the filter, reconstitute the peptide, remove the filter to pull the solution out, put the filter back on, and then push into the final sterile vial.

But the filter still holds some liquid. If the peptide solution displaces the bac that was already in there, that amount gets lost. That's why the two-syringe three-injection method should work better and lets you do everything in the final vial without moving the filter on and off. Inject into the final vial through the filter, wet the filter with bac, push the peptide through, then inject more bac. You only swap syringes around.

In that case there is no real point to wetting, in my opinion. From what I have read, wetting standard PES syringe filters does not improve how well they work. The only real reason seems to be avoiding absorption, so if you are going to flush with bac at the end anyway then wetting first looks unnecessary.

Not for me. Still, I rinse it out right after.

Yeah, items function more effectively if damp.