Filtering already in use Reta after 8 weeks

Good question, I like the thinking but I don't have an answer either. Even if filtering restored it exactly as expected, you'd still have degradation to deal with. Separating the solution and freezing half might give similar results. One freeze-thaw cycle will cause some damage, but I'm not sure how that compares to the vial staying reconstituted for 12-16 weeks. Someone probably has charts showing purity after reconstitution versus after a single freeze.

Why buy and reconstitute that much at only 3 mg a week? It would last 33 weeks or roughly 8 months. I might go as far as 12 weeks but not beyond that. I have large vials too, but my wife and I are using 20 mg weekly.

If bacteria is growing I wouldn't filter and use it. I would just toss the vial. No question. And I'm higher risk than most. I'd get a smaller kit and plan for 10 weeks of use. Save the big vial for later once you increase the dose.

Doing the filtration step over might trap the microbes but would miss the toxins they release, while each extra puncture and move just raises the odds of germs getting in, so it's trading one issue for another. That preservative in the diluent limits bacterial increase after opening but doesn't keep things good forever, and standard advice for a punctured multi-use container points to about a month. When a batch won't get finished in good time a tinier container avoids the hassle, which beats feeling off for several days.

When thinking about a large reconstituted volume, my approach would be to prepare several new sterile vials and divide the liquid into smaller usable portions. At your rate I'd split it into three fresh vials of roughly 33 mg each. Then each one would last 10-11 weeks. Do the same with the remaining large vials only when needed, and don't puncture them until you're ready to divide them. You could keep the unopened ones in the fridge. Once punctured the usual time limit applies.

I don't see how running it through a filter a second time would help with an aged container. The filter removes the microbes themselves but leaves their waste products, which could still cause problems. That means the container might seem okay on the surface while actually being unsafe. There's also the matter of having enough for over thirty weeks at that low weekly dose all in a single container, which is too long to keep one open. For the future, I'd divide it into smaller batches when mixing so they're finished within a couple months. Like others have noted, though, this approach doesn't address the breakdown over time.

The price was really good so buying the larger stockpile made sense to me.

Nothing obvious growing in there now but I'm figuring it might develop over time in the container so I'll definitely put these larger ones aside for future use appreciate the input Understood thanks for the details Might experiment with that approach on the upcoming batch though probably I'll opt for the smaller sizes instead and store the bigger ones away with the rest of my collection thanks I thought the straining process would have handled everything but it's useful to learn this appreciate the update