CommunityReconstitution & Math

Intranasal calculation questions

5 replies5 peopleJan 29, 2026☆ Follow
Summary

How do you figure out spray volume per actuation and calculate intranasal peptide doses when the bottle gives no per-spray info?

Users test actual output by filling a bottle and either counting sprays to empty or weighing successive sprays on a scale. One person measured 0.13 ml per spray after counting; another recommends pre-testing a primed bottle into a syringe. Sterile saline is preferred over plain water for nasal safety, and refrigeration is advised. Calculators exist but require knowing the per-spray volume first.

What this discussion establishesWhere people disagree

Water versus sterile saline for reconstituting the solution

Still open

Whether spray volumes are consistent enough across bottles to skip individual testing

Nothing here is advice.

5 replies · 5 people
SlowCinder14archiveopening postJan 29

I have 10 mg arriving and want to verify the math for nasal use. The problem is the spray bottle. I got one without any stated output per actuation. Calculators all ask for that number. Someone said fill the bottle with 5 ml and count how many sprays empty it. I am not sure if the bottle should be primed first or if the amount per spray is reliable enough to trust without checking.

GreyKettle56archiveJan 30

I tried measuring by spraying into a capped syringe. Even when a bottle claims a certain volume per spray it can differ, so test it yourself. Prime the pump first since that is how you will actually use it. Use distilled or deionized water that has been boiled and handle everything with sterile technique to avoid nasal infection.

CopperAlder51archiveJan 30
↳ replying to @SlowCinder14 (opening post)

Sterile saline is better for keeping the sinuses healthy. Certain bottles deliver 0.1 ml per spray and usually state it. Keep the finished spray in the fridge. Smaller bottles tend to give less volume per spray.

CopperSignal68archiveJan 30

I am going to test this with a cheap scale that reads to 0.001 g. I will fill a bottle with saline, spray, weigh, repeat, and see exactly how much liquid comes out each time.

SlowCinder14archiveJan 31
↳ replying to @BlueTimber78

I located a nasal-dose calculator, filled the bottle with 5 ml, counted the sprays until empty, and got 0.13 ml per spray. The tool then suggested how much liquid to add. I took the dose from the reference site, split it in half, and used one half per nostril.

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