CommunityReconstitution & Math

Reconstitution needle size

6 replies4 peopleNov 3, 2025☆ Follow
Summary

What needle gauge and length works best for reconstituting and transferring between vials?

The original poster has used 30G 25mm needles and is considering 25G 25mm instead. One user recommends 25G 38mm needles for faster flow and reaching the bottom of small vials during transfers. They described combining six 10mg vials into one by sequential transfer starting with 1ml bac water. Another user wondered if sequential transfer might affect peptide stability or cause aggregation.

What this discussion establishesStill open

Whether sequential transfer between vials risks peptide stability or increases aggregation

Nothing here is advice.

6 replies · 4 people
PatientThistle39archiveopening postNov 3, 2025

Hey everyone, I'm about to order needles for reconstitution but not sure which size is best. So far I've used 30G by 25mm, though I suspect 25G by 25mm might be an improvement. What sizes do the rest of you use and what would you recommend?

SlowLantern99archiveNov 3, 2025

I like a 25G needle around 38mm long because it gives faster flow and reaches the bottom of most 5ml or smaller vials when moving liquid between them so nothing gets left behind. I just did six 10mg vials by putting 1ml bac water in the first one then moving that solution to the next vial and so on until everything ended up in the last vial as 60mg total for four 15mg doses at 0.25ml each, and the longer 25G needle made the whole process easy.

PatientThistle39archiveNov 3, 2025
↳ replying to @SlowLantern99

Thanks, 25G by 38mm sounds like the right choice.

WarmMeadow68archiveNov 3, 2025
↳ replying to @SlowLantern99

I've been interested in trying that approach but never got around to looking into the details because life keeps getting in the way with work and everything else.

SlowLantern99archiveNov 3, 2025
↳ replying to @WarmMeadow68

Same as with lots of other topics, I've meant to write something up about it because it seems useful to plenty of people, but the usual daily chaos always interferes.

WarmMeadow68archiveNov 5, 2025
↳ replying to @SlowLantern99

I was checking into this method. Wouldn't moving the solution from one vial to the next potentially affect how stable the peptide stays or raise the odds of aggregation? I came across some information about it.

IronThistle42archiveNov 5, 2025
↳ replying to @SlowLantern99

A-ha, now I have an idea for what to do with my leftover 10s and 20s!

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