SS-31 Dosing Sequencing Side Effects and Mitochondrial Repair Before MOTS-c

I am in my early forties and have used Mots C without SS-31. It gives a clear energy lift and works well for me. My husband who is in his fifties has the same experience. SS-31 may not be necessary for everyone. If the energy boost does not show up then a round of SS-31 could be tried next.

I am thirty-two and began Mots C after only a few SS-31 doses. The combination with NAD+ gives better energy for me. SS-31 alone has not stood out yet, though I have only used it a handful of times because daily morning injections are easy to forget. I did NAD+ three times a week for a month before starting Mots C and liked it. I plan to keep both going until supplies run low then drop to weekly NAD+. I settled on four milligrams of Mots C because the math worked better with the vials I have.

Thanks for the reply. I am almost forty-seven. What weekly amount are you using?

I saved that protocol write-up when I came across it. I have enough on hand to run it but will probably wait until later in the year after finishing some other peptides. Looking forward to seeing what Mots C and NAD+ do together.

We began at one point five milligrams three times per week. We also run NAD+ twice a week so energy stays steady from Monday through Friday.

Are you referring to the Mots C or the SS-31? How long does your cycle run?

Yes, Mots C. We have not tried SS-31. We have been on it three weeks now and it has made a big difference. I feel for anyone who does not get the energy lift from it.

I would like more detail on those amounts. What studies or milligram-per-kilogram conversions are they based on? Not judging, just curious.

I am not sure why the question is aimed only at me when five milligrams comes up often from others here. The common protocols and their sources are listed on the reference site. I already posted that earlier so I will not repeat it.

My first message said I was not coming at you. Your choice on dosing. I did check the reference list. The question remains whether those entries come from user reports, studies, or both. You mentioned pinning five milligrams daily and sometimes ten depending on how you feel, so that is why I asked you. I would like to see the source showing ten milligrams daily gives clear benefit. I enjoy your posts and would like to discuss nootropics more.

No human data sets an ideal Mots C protocol. Converting mouse-study doses to human equivalents gives amounts well above five to ten milligrams. That does not mean anyone should follow the animal-study numbers directly. The studies are on the reference site and searchable on PubMed. I do not have time to link and summarize them all here.

Good to know it is not hard to find and costs less than most other peptides.

When you say units it is hard to follow. Do you mean ten milligrams or point one zero milliliters? What was your reconstitution?

How many milligrams of Mots C in each injection?

So one milligram. Thumbs up.

Was the vial already liquid or did you add bac water or something else? How much? For example if I had a ten milligram vial and wanted five milligrams I might add one milliliter bac water and inject point five milliliters. That would be a ten milligrams per milliliter concentration. Units or milliliters only make sense once the concentration is known.

Correct. Thinking maybe moving up to fifteen units.

No, I added bac water, one milliliter.

Good. It is simpler to talk about doses in milligrams rather than units or milliliters so concentration does not need explaining each time. You could say you tried one milligram three times a week. That is a cautious start, good luck.

What week did you first notice it working?

I went ahead with bigger quantities on my end, despite many folks saying to start low, so your method looks good to me. People have different reactions, meaning you could require moving toward standard amounts before effects appear.

From a dosing view it is clearer to state the actual peptide amount rather than units. Ten milligrams per milliliter would give one point five milligrams in a fifteen unit injection. If the same ten milligram vial had been mixed with two milliliters instead the concentration would be five milligrams per milliliter so fifteen units would be point seven five milligrams. Same volume, different amount.

Do you usually add two milliliters of bac water to a ten milligram vial?

This matches how I take mine except I run SS-31 and NAD+ daily without skipping weekends.

SS-31 is not meant to give energy. It is meant to repair mitochondria before starting Mots C. It should be taken first if you are over forty.

My introduction to this sequence came from some research I stumbled across online. One substance works like a repairman for the power-producing structures inside cells while the other acts as a signal to improve how the body switches fuel sources. The proper sequence matters because the repair step needs to happen ahead of the signaling step. The initial compound attaches to a key fat in the inner membrane to restore its shape and allow smoother energy output. People often apply it over a stretch of weeks when dealing with low energy or advancing age. The follow-up compound copies the effects of movement by switching on a regulator that favors fat use and better sugar response. Applying the signal before the underlying fix is complete resembles forcing a damaged motor to work harder. Although both can be taken at once the preferred order helps avoid sudden energy dips or mismatched responses.

Something went wrong on my end too. Can't tell what triggered it.

I suspect a few things might be going on, maybe all at once. For one, I skipped any protective compound ahead of my protocol. Being 49 after years of nonstop excess, my cellular energy factories are probably worn down. On top of that, glucose got too low from stacking the peptides with strict carb cutting. Or perhaps my system is just adjusting to a fresh fuel pathway triggered by the peptide, and the shift is creating these effects. Tough to pin down the exact cause. Next round I'll include the protective one plus the other booster and observe the difference.

Units meaning ten units equals one milligram, correct?

That is correct

That seems like a good amount to begin with. I might go with something similar myself.