Syringe gauge selection and sterile filtering loops for vial reconstitution

Up to this point I have not done any filtering yet I am considering trying it with the batches ahead. What should be done about the remainder blow air through it rinse away using the solution or just ignore it.

I draw a small volume of air into the syringe either ahead of or after the filtration to clear the line. Most times I handle that part afterward by taking the syringe off the filter first, sucking in some air, putting it back on, and forcing everything through.

I add just a portion of the bacteriostatic water to the starting container. That creates a stronger mixture I pass through a filter into my clean sterile container or sometimes a pen cartridge. Then I run the leftover water through the filter too. This approach lets me hold on to more of the peptide.

I've tried both approaches but these days air seems better to me. The leftover volume trapped in a 13mm setup comes to roughly 0.15 milliliters which at that strength equals a small quantity left behind in the filter. Another option is to reconstitute a 60mg amount using 2ml total bacteriostatic water to reach the target strength. Start by adding 1ml for the first step and run it through the filter then use the remaining water plus the trapped volume to wash out the starting container. Hardly any of the substance ends up in that wash so there's no need to clear the air. The difference shows up more with bigger vials or pricier substances that run several times higher per unit. The wash approach takes one filter two barrels and four needles altogether.

My acquaintance is following that approach too. Maybe I'll select it. I'll proceed with the upcoming containers, not certain which one to pick. Could go for that particular one. Calculations show one additional six milligram dose for each of the current batches. Perhaps two instead.

Have you gone for the WW 140mg yet? Availability continues. Temptation lingers because the order tool still marks it ready.

The deadline stays active right up through the twenty-fifth. So far I've covered payment on eight kits, though ten would make for a nicer even total. 🤣

This ought to last quite some time. I've been mulling it over recently. Time to go for it. It shows up just below those two I bought earlier. Reach out if you want the details on that. Checked and spotted a name but unsure of the person. Now it's clear in the order listing.

Yeah, skipping this chance just isn't happening for me. I've mulled the whole thing over endlessly. An R140, that's wild. Our last stretch took six weeks with a couple of R40 bottles, and the count shows one left, yet it seems we'll need two extra.

This situation has me baffled. Apparently the thing was discovered, a provider was inquired about, it was checked out and observed, and now it's apparent once more. 😂

It took around nine minutes using just a single milliliter of bacteriostatic water, if memory serves, as a quick test. I'm thinking about running the solution through a filter into a five milliliter container. At the amount I'm using each week that container would last roughly two months. Dividing it across several containers could work better if the stability lasted longer. I overlooked adding the container expense into the total for filter materials, so the overall supply cost rises, and it goes up more with an added filter on the vent needle. The alcohol pads contribute a very small extra amount when counting every detail.

I just ordered a kit. Turns out I can't stand myself either way!

Man we both come across as total lost causes here.

😂 Those two extras will bring me up to roughly ten kits holding fourteen grams of Reta 😂

I skip the filter on the vent. Air flows out more than it comes in.

WW?

Got a supply here that covers five milligrams weekly across nine years. It matches half the time left on my clock per those life expectancy charts. Achieved the goal I set for living.

Turning forty soon means another ten years before I could reach the upper end of what the AI projected from all my collected numbers, even at the highest experimental amounts of those compounds. Looks like we share the same lack of prospects.

That clears things up nicely. I am hopeful about securing the wings ahead of the twenty fifth.

I got interested in who provides it, but then noticed the details on the ordering page. My mind went blank once more. Age is catching up with me. Keep it undercover and never say the actual source. It's really bad, actually it's not. You'll see. Could you include me on that group? I've been going for about five and a half years using twelve milligrams weekly shared with my spouse, but now it's alarming. I expect to still have nine remaining without trouble.

People talked extensively on the subject since those smaller containers would require manufacturing adjustments that would push GB expenses past what the company could accept. This maker earns more of my confidence than the others do. I intend to split my own batch through filtering so it goes into smaller containers and becomes simpler to handle.

Transferring solution into another small container involves some expense on supplies, yet this seems like an unproductive struggle that's acceptable but could leave you isolated. The arriving concentration for those larger dose containers differs from the figure you mentioned, and I don't know how you arrived at that value.

I joined that chat earlier on. Considered picking up a bundle just so I could attach a complex interface to the unit, but the total cost ended up being more than I could handle.

The 30mg/mL is for tirzepatide, and the maker is not selling retatrutide yet.

Right.... so where is the maker saying Reta is limited to 25mg/ml?

During the studies, much as with the tirzepatide experiments, those pens were restricted to a maximum of half a milliliter. Hence the solubility reaches a peak of 30 milligrams per milliliter for tirzepatide and 24 for retatrutide.

Sent you a DM

I wonder if you've sampled their offerings yet. That conversation with the founder seemed striking, much like the factory recordings. I've felt the urge to test them out from that point onward. The complete bundle is beyond what I can manage, though I'd prefer just one piece to tinker with should someone opt to sell it alone.

Picked up some Mots recently from that source. Not used any yet, though the tests and documentation look solid. Appreciate how they handle everything in-house. Stashing my Reta away for later when things get tough.

Any idea what one might be valued at? Direct messages are fine if you like that better.

Sure the dude who weighs everything by the gram has the right idea.

Seemed like there might have been a tapping noise.

I always filter the vent needle. I can easily overstate what the wipes add up to. That amount covers what each container is worth to me. Even so, every item together with the small bottle sitting nearby falls well short of a big monthly total.

I'm going with that idea myself. Already placed an order for the set. Delivery is going to take some time. Looks like we're both doing the same thing.

I'm not seeing any problems with this approach. My plan involves starting with three milliliters to handle most of the dissolving, shifting the solution across, and following up with two milliliters returned to the first container for a rinse step before transferring that liquid into the second container. Altogether that reaches five milliliters at one hundred forty, which looks acceptable. Though I could end up hesitating and instead splitting the process across two separate three-milliliter additions with a ten-milliliter container.

I was playing around with some math on the dosing. Considered a 6 milliliter volume inside a 10 milliliter container to deliver a 6 milligram injection using 26 units. Comes out to roughly two dozen doses. That covers three months' supply for both my spouse and me, each doing the 6 milligram daily amount without changing the current level. Seems like I calculated correctly. Wondering about the overall filled quantity though.