CommunityInjection Technique

First time, Last Minute Questions and Checklist

11 replies8 peopleMar 11, 2026☆ Follow
Summary

What equipment choices and steps matter for safe first-time reconstitution and filtering of tirzepatide?

Users share personal setups for filtering reconstituted peptide into sterile vials, including syringe and needle sizes, filter handling, and volumes. Several recommend pre-wetting the filter, using one filter per vial, and adjusting concentrations to keep injection volumes low. Opinions vary on needle gauges for drawing versus injecting and on whether filtering is essential versus optional.

What this discussion establishesWhere people disagree

Whether to always filter or if single-vial reconstitution without filtering is acceptable for beginners

Still open

Maximum comfortable subQ injection volume and whether batch reconstitution followed by later filtering is advisable

Nothing here is advice.

11 replies · 8 people
WryQuill22archiveopening postMar 11

Hi everyone. It's nearly time for me to start. Would anyone mind answering a few last-minute questions? I'm only setting up for Tirz. I also listed my shopping items below in case I missed anything. Should I use a new filter for each sterile vial? I plan to split 30 mg across two or three vials and freeze the extras so no vial is used longer than four to six weeks. Are 3 mL syringe barrels fine? How many barrels should I use per filter run? One person just pushes air through the filter at the end. The guide uses only one barrel but I'm concerned about contaminating the BAC vial even with just 0.1 mL of air from the same barrel I used for reconstitution. I'm probably overthinking it. Is there a real advantage to using two different needle lengths for filtering instead of just the longer one? The guide lists both lengths and uses four short ones plus one long one. Is it a bad idea to use a vial opener to pull out the last drops?

PlainAlder11archiveMar 11

For my syringes I keep the 3 mL ones for reconstitution. I use 0.5 mL and 1 mL 31 G 8 mm syringes for injections. 25 G feels large to me. My wife would not be happy if I used a 25 G. This is for Reta.

SharpCinder28archiveMar 11
↳ replying to @PlainAlder11

You get better placebo effects with 25 G harpoons for the less common peptides. For intramuscular glutathione I use 27 G and it is not bad at all.

PlainAlder11archiveMar 11
↳ replying to @SharpCinder28

My friends who lift and use testosterone including my son use 23 to 25 G one-inch syringes. I call them horse needles.

GreyHarbour42archiveMar 11
↳ replying to @WryQuill22 (opening post)

Thanks for posting this. It looks like a solid starting point for my own research. There is more involved than I expected. Good luck with your setup.

WryQuill22archiveMar 11
↳ replying to @PlainAlder11

The 25 G is only for reconstitution. I am getting separate insulin syringes for the actual injections.

LevelTimber64archiveMar 12

I use a 13 mm filter. I have read that 4 mm is rated for up to 1 mL while 13 mm handles 1 to 10 mL. Do not forget to account for the liquid held back in the filter. That is about 0.07 mL on a 4 mm and 0.28 mL on a 13 mm so either adjust your BAC volume or plan to be slightly over. If you get a tray for filtering make sure it is stainless steel. I thought I had everything ready the first time but once I laid it all out I panicked and could not remember the steps, so I followed a video instead.

WryQuill22archiveMar 12
↳ replying to @LevelTimber64

Thanks, that is new to me. I will get the 13 mm size next time when I need more vials anyway. Glad I did not order a hundred of the smaller ones. It will take a little longer but I will just filter 1 mL into each vial for now.

ClearTimber11archiveMar 12

I stopped using 25 G needles for reconstitution. The self-healing cap stopped sealing properly and allowed air to move in and out while drawing or pushing air. Since then I have switched to 30 G. It is slower but safer.

GreyWillow12archiveMar 14

Hi everyone, another complete beginner here. I am only now learning about filtering. Is this standard practice or just personal preference?

GreyHarbour42archiveMar 16
↳ replying to @GreyHarbour42

If you are just starting and plan to reconstitute one vial at a time, can you skip filtering? Would one larger syringe for reconstitution and separate smaller ones for dosing be enough?

GreyLantern40archiveMar 16
↳ replying to @GreyWillow12

It is personal preference but I feel strongly that I would not inject anything I had not filtered. There are photos showing particles, bugs, or hair in reconstituted peptides. The 0.22 micron filters are fine enough to remove bacteria. Sterility can be an issue so filtering makes sense to me.

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