I'd appreciate a list of the supplies required to begin filtering peptides myself. Maybe something like an online shopping list.
Supplies list for filtering
Summary
What supplies are needed to start filtering peptides at home?
Forum users point to searching existing threads for lists rather than new requests. One detailed reply breaks it down to three core items: luer-lock syringes in 3 ml size, matching needles in two gauges for drawing and dispensing, and 13 mm PES sterile syringe filters. Others note that starter kits exist but cost more per use than buying components separately, and some users prefer seeing physical items over lists alone. Replies also compare luer-lock versus luer-slip connections for safety under pressure.
What this discussion establishes- Core items are luer-lock syringes, 25G and 30G needles, and PES 13 mm filters
- Luer-lock preferred by some for higher pressure during filtering
- Kits can serve as visual starters but are more expensive per vial than sourcing parts individually
- Search function and prior threads are repeatedly recommended over new requests
- Some users value holding actual items to match cheaper alternatives later
Whether luer-lock syringes are strictly necessary or if luer-slip works fine without issues
Still openAvailability of kits or components for users outside the main shipping regions
Nothing here is advice.
15 replies · 10 people
Search works really well here, and the tips in my signature can help if you get stuck. There's a long thread with lots of details on what others use and how they do it.
I saved one of these lists earlier since the topic comes up often. A few things like the 5 ml syringe might not be essential, but they come in handy sometimes.
↳ replying to @BlueSignal97
I gave the search a try but didn't locate the details I wanted.
Really just three main items. First, a 3 ml luer-lock syringe so you can switch needles easily, one for drawing and another for pushing through the filter. Larger capacity makes it simpler to handle a whole vial at once. Second, luer-lock needles, 25G 1.5 inch for drawing because they reach the bottom without bending and give good flow, plus optional 30G 0.5 inch for dispensing to reduce coring. Third, a sterile PES syringe filter, 13 mm diameter.
I looked at that kit earlier. It only handles five vials though, which works out expensive per vial if you're just learning. There's also a version with the smaller 13 mm filter.
↳ replying to @NorthQuill51
I'm looking into this too, though options seem more limited where I am.
↳ replying to @PlainAlder11
Quick question on that kit: it lists two 30 ml bottles of BAC water but only covers five vials. Too bad about the shipping limits.
↳ replying to @NorthQuill51
Any specific reason most people go with luer-lock syringes instead of luer-slip? The advice always leans toward lock, yet I've used slip without problems. Is it just preference or does lock handle something better?
↳ replying to @LevelBramble14
You'll want the version without sodium chloride. The needles I use work with both types.
The two styles work the same way overall, but lock uses a screw mechanism for a more secure fit while slip is just a push-on connection meant for quicker jobs. Slip can come loose so extra care is needed, though both get the job done.
↳ replying to @ClearAnchor21
I think lock is viewed as safer because of the pressure involved in filtering. Interestingly the filter itself has lock on one side and slip on the other, probably due to different pressure levels.
The video looks useful, thanks. I was wondering what you meant about not liking lists and how having the actual items helped more than just seeing a list of them.
↳ replying to @SharpPebble53
Lists are okay. I just prefer getting the real items in hand first. For instance the kit gives you four 3 ml luer-lock syringes or eight 25 g needles of a certain length. Seeing and touching them lets me match the exact same things cheaper from other places. Most people do fine with lists, but I'm very visual and like laying everything out to compare. The video helps too except for the part about adding a filter to the vent needle. It doesn't seem necessary if you're already adding air to the vials anyway. Maybe it's just to sell extra filters.
↳ replying to @SlowBeacon16
That makes sense for comparing while shopping. I'm sure once I actually try filtering it will become clearer too.
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