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Mitochondria Peptide Protocol Questions

12 replies6 peopleMar 26, 2026☆ Follow
Summary

What sequencing and dosing works for SS-31, NAD, MOTS-c and Tesa together for mitochondrial support?

Users describe a 14-week staggered approach starting with NAD alone, adding SS-31 next, then switching to MOTS-c. Tesa is taken at night, often alongside ipamorelin. Injection-site discomfort with NAD varies by person, concentration and site; some report it fades with shoulder injections. Results with Tesa plus ipamorelin are described as subtle but positive at standard doses.

What this discussion establishesStill open

Whether the staggered protocol is more effective than simultaneous use, and how noticeable the benefits actually are for most people

Nothing here is advice.

12 replies · 6 people
PatientSignal25archiveopening postMar 26

My shipment should arrive soon and I'm eager to start. I found a plan that begins with SS-31, then layers in NAD and MOTS-c. I've read NAD can cause extended stinging at the site. Also wondering if adding SS-31 and the newer Tesa together for a first trial is fine, since I don't know how two new items at once will feel. What daily amounts is everyone using? I'm considering SS-31 at 2 mg each morning for the first four weeks, Tesa at 2 mg daily, then after week four adding NAD and MOTS-c at 2 mg daily. All shots first thing in the morning seems reasonable. I'm already on 2.5 mg reta weekly and 1.5 mg klow daily into the sore area. Any thoughts?

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PlainPebble65archiveMar 26

Forum searches turn up a 14-week schedule several people follow: NAD by itself for the first two weeks, SS-31 added for the next four, then MOTS-c swapped in for the remaining eight. Tesa should be pinned at night and ideally with ipamorelin.

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PatientSignal25archiveMar 26
↳ replying to @PlainPebble65

Have you used NAD before? I keep hearing it stings for a long time afterward. Also, Tesa at night? I'll check that out.

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PlainPebble65archiveMar 27

Yes, I'm finishing the last weeks of that same schedule. By sting I take it you mean soreness where you inject. Several peptides can do that depending on how much you use, how concentrated it is, where you pin and your own reaction. It bothers my wife more than it does me.

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AmberFenwick97archiveApr 4

Tesa and ipamorelin together give subtle but solid results at the usual amounts people recommend.

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PatientSignal25archiveApr 4
↳ replying to @PlainPebble65

I switched to injecting right into the shoulder where the rotator-cuff pain is. The stinging stopped and I no longer have to dilute it.

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PatientSignal25archiveApr 4
↳ replying to @AmberFenwick97

Someone told me 2 mg Tesa every night before bed. Does that sound correct? Hoping it works out.

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ClearAnchor21archiveApr 4
↳ replying to @PatientSignal25

I've been using NAD for over a month at 100 mg every other day. No stinging for me, but I add quite a bit of bac water now (80 units per 100 mg) after one vial crystallized from being too concentrated. I'll probably use less water on the next vial since 80 units feels like a lot to inject.

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AmberFenwick97archiveApr 5
↳ replying to @PatientSignal25

People are getting results with 5 mg Tesa plus 5 mg ipamorelin.

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ClearMarble44archiveApr 5
↳ replying to @PlainPebble65

Does anyone know why the protocol avoids stacking SS-31 and MOTS-c at the same time instead of just stopping one and starting the other? I've got an ultra-endurance event coming up and am looking at this approach.

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ClearMarble44archiveApr 5
↳ replying to @ClearMarble44

Thinking about it and running the numbers, I'm guessing cost is the main reason.

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QuietKettle56archiveApr 17
↳ replying to @PlainPebble65

How did the protocol feel for you? Thinking about starting it at some point.

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QuietKettle56archiveApr 18

Sorry, I already have the protocol details. I should have been clearer. I meant how did it feel overall? Did you notice benefits? Was it worth doing?

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