Participants describe personal regimens ranging from daily 1 mg to 5 mg several times per week, frequently cycled with NAD+ or after SS-31. Modest gains in steady energy, workout stamina, or reduced lethargy are noted by some while others observe no clear change. TMG is discussed mainly as a methyl donor when NAD+ is used, with warnings about possible heart effects if omitted and reports of bloating. Injection-site itch or lumps appear regularly, prompting suggestions for histamine control. Questions remain about continuous versus intermittent use and how prior SS-31 exposure or exact dose alters results.
What this discussion establishesextent of noticeable benefits and whether TMG omission poses real heart risk
Still openoptimal continuous-versus-break protocols and how results vary by prior SS-31 use
Nothing here is advice.
I'm seeing lots of different advice no matter where I look.
Began with two kits at 5 mg on Monday Wednesday Friday for four weeks, then switched to 10 mg once weekly for fourteen weeks. The plan was to stop after sixteen weeks but two vials remained so I kept going. Before starting MOTS I had done a round of SS-31 and noticed more from that.
Just to note I never tried ss31. I was using 10 mg of mots c three times each week for four to six weeks. Nothing stood out enough to make me want another round. Maybe doing ss31 first really changes things the way some claim.
I hear a lot of people suggest trying ss31 first for various issues. It sounds pretty strong.
Mots gave me awful injection site reactions so I stopped. SS31 on the other hand helps me break through plateaus. When I first used it I also slept really well.
I should give ss31 a try. What does ISR stand for?
My shot area showed redness a bumpy rash puffiness hurt discoloration an urge to scratch bacterial issues a pocket of pus leaking fluid dead skin going unconscious and passing away.
I was only joking about the coma and death part. I just realized I was starting to sound like a drug commercial so I figured why not.
I shared this in another place but it lays out a mitochondria protocol along with the reasoning for the doses. I am just finishing week three.
This catches my interest because it has a clear end point. As someone who likes details I appreciate the reasoning even if it turns out to be overstated.
ISR is short for injection site reaction such as irritation, welts, itching or pain. I tried Mots once and it burned badly then left a large red raised itchy area. I had to use a cold pack and antihistamine cream before it settled. I planned to add more bac water next time but went on vacation for two weeks and when I returned the vial looked cloudy so I threw it out. Makes me wonder what was in it or if the other vials are affected.
I must have too much free time because after reading that protocol I immediately wanted to start it.
I'm feeling anxious about giving it another shot. I got a kit and the first container had ruined filters yet I went ahead with it anyway. It burned intensely right away and swelled up into a big raised bump. I put on an ice pack plus some antihistamine lotion to ease the reaction. After being away for a couple weeks I returned to find it looking murky. This time around I'm cautious and plan to double check that the filtering steps get done right. When a product causes burning for most people do the tricks used with another item help like a longer needle or numbing cream. I've got magnesium oil but it left my skin too slick to hold a pinch steady. I wiped it down with an alcohol pad but it stayed slippery. Should I try injecting around the hip area or other spots?
I was content with 5 mg per week split into two shots. I am five or six weeks into ss-31 and have not noticed any effect. These two compounds seem to hit people differently. Optimal dosing probably varies a lot based on individual physiology.
I like the mots I have. The vials are 10 mg so I am running 10 mg per week for four weeks. I add 1 mL of bac water and inject 33 units on Monday Wednesday Friday. Mainly doing it for long term mitochondrial health but it does give a subtle clean energy boost for about half a day.
Now I have more questions after reading that.
I do not use that particular source but I still find the product a bit spicy or burning for a few minutes at the site and sometimes get minor bruising. It is not a big issue for me but it does happen. Not sure about that one source.
I got the large welts and intense itching from several different sources. The workaround that has worked for me is to have KPV ready in a separate needle. I inject the Mots first then immediately do 500 mcg of KPV right next to it but not into the same spot. I have done this at least six times. I still get a knot but no itchy welt. I follow the standard protocol of 5 mg three times a week every other day.
Have you tried the KPV first before the mots? I wonder if that order might reduce the sting better. I used lidocaine cream fifteen minutes before injecting today and it helped a lot. Thanks for the tip. Now I need to get some KPV.
Good luck to everyone researching mots c. Not trying to discourage anyone but it did not do much for me. I was using 10 mg at a time three days a week. No pain or site reaction but also no clear benefits. I had not done ss31 beforehand and maybe my mitochondria are already in good shape though that seems unlikely.
I tried 2 mg of MOTSc in 0.2 ml mixed with 0.1 ml bac water into the glutes. That seemed to work well. I will keep testing different sites and dilution levels.
My issue was that the MOTS did not hurt at first. The itching and big welt showed up hours later or the next day. Adding lidocaine to the shot once did not help. Pinning KPV first should work just as well. I have been injecting the Mots then leaving the needle in place while adding the KPV right beside it before pulling both out.
I see that makes sense. When I tried it the sting started right away and was very painful with the welt forming at the same time. Antihistamine cream and a frozen pack brought it down almost completely. That is why I would try the reverse order you described. I will definitely use lidocaine cream ahead of time.
How is everyone's progress going? I would like to hear updates. I have become a bit hesitant and would appreciate any feedback. Thanks in advance.
Seconded. Several people here mentioned they were following the NAD plus SS-31 MOTS-C protocol and should be on the MOTS-C portion by now. I would like to hear how that is going since I am still on the SS-31 stage.
I need to get some of that. How does it feel?
I am trying to wait two full weeks before deciding for sure because I want to rule out placebo. So far I feel better. Not as awake as before I started Tirzepatide at the end of July but much more awake than recently. I do it right before work so I stay alert through the day and have started working out again.
5 mg Monday Wednesday Friday in the morning. This is week two. I get a little itchiness for ten to fifteen minutes and slight redness at the site. Nothing that really bothers me. It has not hurt my two hour cardio sessions each morning.
Wow two hours that is impressive at least to me.
That is great news.
Things are looking good at the moment. This option has me optimistic since constant tiredness has grown really tiresome. Permanent exhaustion would be acceptable if it brings peaceful nighttime breathing, an appealing look and sensation, plus total delight. Still a quick fix for the weariness sounds perfect.
What happens after week fourteen?
I am on the mots-c portion of the SS-31 MOTS-C NAD+ protocol. I have 40 mg in 4 ml saline in a nasal spray bottle and do two sprays in each nostril before running each morning. I had the nasal version from an earlier study. It worked well and I was confident I would not react to an injection so I kept making more spray. It seems effective as I notice results. My A1c has dropped noticeably and I can run a bit faster than usual. I plan to test fasting insulin and glucose after finishing the protocol to calculate HOMA-IR.
I had been looking into the NAD+ SS-31 MOTS-C protocol and reading other threads but none of them mentioned taking Betaine with the NAD+. Not sure if I can name a specific site here but there is a quote from one warning about depleting the methyl pool and needing betaine to avoid serious issues from that depletion.
Nice find. I cannot wait to casually mention homocysteine around someone who is really into NAD+.
From what I have seen anecdotally some people recommend pairing NAD+ with glutathione because it is a strong antioxidant.
My wife and I both like mots as part of our morning pre workout routine. We are running it alongside our workout plan of four days on one day off. I use 3 mg and she uses 2 mg on that schedule.
I thought it was common knowledge that you need to stack TMG or another methyl donor with NAD. Maybe it is not so I will keep mentioning it.
The protocol document I downloaded does not mention it at all. You would think they would flag that detail because it can lead to heart issues.
I think the heart issues and betaine deserve more attention. Someone else mentioned Trimethylglycine/Betaine Anhydrous so I am going to check into that. I will try sharing the article link in case it helps, though I hope that does not cause any problems.
Do betaine and trimethylglycine refer to the same substance?
TMG is also called Trimethylglycine and is the same as Betaine Anhydrous. Betaine HCL is a different form.
Glad this came up. I am about to start NAD+ once mine arrives and had not heard of that before. Looks like I will be reading up on it now.
Someone covers this in detail with smaller starting amounts around 300 mcg and lists many different protocols. I am experimenting myself. My mitochondrial markers from bloodwork looked fine. I am using 2 mg and felt like superman at work one day with clean energy, though other times nothing. I have the materials ready for the SS31, NAD, MOTS-c protocol and it seems SLU-PP-332 fits into the mitochondrial approach.
What benefits did you see from MOTS-c?
Unfortunately the effects were very small for me. Maybe I was already optimized. Your results could turn out better though. Good luck.
Most people notice an increase in clean energy rather than the short jittery feeling from pre-workout. The benefits include better fat metabolism that mimics exercise, improved insulin sensitivity for blood sugar control, faster muscle recovery, better cognitive function, and possible protection against age-related issues.
I am doing 5 mg three times a week on Monday, Wednesday, Friday on an empty stomach in the morning, usually thirty to forty-five minutes before exercise.
I agree a methyl donor is useful with NAD+ but I would be careful with that explanation. Science has not fully worked out the exact details of NAD+ metabolism yet.
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